Proline biosynthesis in M. ruber: work done during the winter and spring of 2015

This is the first of a three-part video series.  It was made in May of 2015 and it represents the work of my BIOL375/Molecular Genetics class  and several students working in my lab at Augustana College.  In this video, I describe how we arrived at the prediction that M. ruber follows the usual sequence in synthesizing the amino acid proline from glutamate. We use the bioinformatics programs and online lab manual associated with GENI-ACT (an NSF-funded project).

This is the second of a three-part video series related to proline biosynthesis project in the organism M. ruber.  In this video, I describe the complementation assay.  Complementation is a functional genomics counterpart to a genome annotation project. It provides the benchtop/experimental evidence to support the annotation predictions.  The protocol for the complementation assay is described on the GENI-Science site.